Efeito do omega3 na cardiomiopatia de camundongos com distrofia muscular em fase avançada da doença
Brasil – omega 3 foi administrado em camundongos com distrofia muscular com 8 meses de idade. Os animais tratados apresentaram redução das citoxinas inflmatórias e fibrosantes, redução da CK e melhora de alguns parâmetros funcionais.
O resumo em inglês pode ser lido abaixo:
(Neuroscience Meeting, 2014) Effects of omega-3 therapy in the cardiomyopathy of the mdx mice, at later stages of the disease
A. F. MAURICIO, J. A. PEREIRA, H. SANTO NETO, M. J. MARQUES – Brazil
Duchenne muscular dystrophy is the most common and severe dystrophynopathy in childhood characterized by absence of dystrophin, with progressive muscle wasting and cardiorespiratory failure. In the absence of dystrophin there is sarcolemma instability, increased calcium influx and myonecrosis. Cardiomyopathy is one of the most frequent causes of death in DMD. In the mdx mice model of DMD, signs of cardiomyopathy are first seen around 9 months of age. We investigated the effects of omega-3 therapy in the mdx cardiomyopathy, at later stages of disease (13 months of age). Mdx mice (8 months of age) received fish oil containing eicosapentaenoic acid and docosahexanoic acid (300mg/kg via gavage, 3 days a week), for 5 months. Control mdx received nujol in an equivalent dose and period. Control mdx showed elevated (3 times) serum levels of CK-MB, an indicator of heart necrosis, compared with normal C57BL/10. Omega-3 reduced CK-MB (119±20 UI in mdx-nujol vs. 86±20 UI in mdx-omega-3). In control mdx, electrocardiogram analysis indicated alterations in the amplitudes of some waves, a decrease in the R/S ratio and a significant increase in the cardiomyopathy index. Omega-3 ameliorated some of these heart functional parameters. No changes in heart fibrosis area were seen in omega-3-mdx, with higher levels of fibrosis in the right ventricule (16±3% in mdx-nujol vs. 13±3% in mdx-omega-3). The levels of TNF-a (proinflammatory cytokine), TGF-b (profibrotic factor) and metaloproteinases(MMP)-9 and MMP-2 were all increased in the heart of control mdx in comparison to normal C57BL/10. Omega-3 significantly reduced the levels of TNF-a (1.5±0.4 in mdx-nujol vs. 1.1±0.03 in mdx-omega-3) and MMP-9 (1.3±0.1 in mdx-nujol vs. 1.1±0.09 in mdx-omega-3), with a tendency to reduce TGF-b (1.9±0.3 in mdx-nujol vs. 1.7±0.4 in mdx-omega-3; p > 0.05, Anova). The present results demonstrate that omega-3 is effective against cardiomyopathy in the mdx mouse, at later stages of the disease, being able to improve functional parameters and to regulate molecular markers (TNF-a, TGF-b and MMPs) of dystrophy progression, therefore deserving future investigation in DMD clinical trials.
Fonte: http://distrofiamuscular.net/principal.htm
